https://dspace.piwet.pulawy.pl:443 Repozytorium cyfrowe DSpace zapisuje, przechowuje, indeksuje i udostępnia cyfrowe materiały. Tue, 21 Jul 2026 13:32:08 GMT 2026-07-21T13:32:08Z https://dspace.piwet.pulawy.pl/xmlui/handle/123456789/963 Dostępność białka paszowego w Polsce w kontekście wykorzystania soi genetycznie zmodyfikowanej oraz surowców alternatywnych Mazur, Małgorzata; Sieradzki, Zbigniew; Król, Beata Biał ko jest jednym z naj waż niejszych składni ków pokar mo - wych w ży wieniu zwierząt gospo darskich, warun ku ją cym pra- wi dłowy wzrost, rozwój, pro duk cyj ność oraz zdrowot ność or- ga ni zmu. Jego podsta wo wą funkcją jest udział w bu do wie tka- nek oraz syntezie enzy mów, hormo nów i przeciw ciał. W prak - ty ce zootech nicznej odpo wied nie zaopa trze nie zwierząt w ten skład nik jest jednym z klu czowych elemen tów deter mi nu ją - cych efek tyw ność pro duk cji zwie rzę cej. Thu, 01 Jan 2026 00:00:00 GMT https://dspace.piwet.pulawy.pl/xmlui/handle/123456789/963 2026-01-01T00:00:00Z https://dspace.piwet.pulawy.pl/xmlui/handle/123456789/962 GMO w łańcuchu żywnościowym Sieradzki, Zbigniew; Mazur, Małgorzata; Król, Beata Thu, 01 Jan 2026 00:00:00 GMT https://dspace.piwet.pulawy.pl/xmlui/handle/123456789/962 2026-01-01T00:00:00Z https://dspace.piwet.pulawy.pl/xmlui/handle/123456789/961 First identification of orf virus from an outbreak of contagious ecthyma in Polish sheep Larska, Magdalena; Nowakowska, Agnieszka; Pękala-Duda, Urszula Contagious ecthyma (CE) is a skin disease of goats, sheep and some other domesticated and wild ruminants worldwide, including Poland. The infection is caused by the orf virus (ORFV) of the Parapoxvirus genus. It is characterised by the appearance of skin lesions in the form of papules and pustules, most often in the area of the mouth and nostrils, hooves and genitals. The disease causes economic losses, especially in countries with high sheep and goat production. In Europe, the threat posed by this disease is rather underestimated, but following the re-emergence of foot-and-mouth disease (FMD) in the region in 2025, there is increased vigilance and a need to have differential diagnostic methods proven and ready. Material and Methods: DNA was extracted from lesions on mouth and hooves of sheep. The animals were on a farm located in southwestern Poland on which FMD was ruled out. A PCR was performed using two pairs of specific primers for detecting vIL10 and GIF gene fragments which were then sequenced. Results: Sequencing confirmed ORFV infection in all tested sheep samples. The sequences obtained for Polish viruses were also highly homologous to other parapoxviruses, indicating that the method used may be applicable for broad diagnosis of parapoxvirus infections not only in ruminants. Conclusion: This is the first molecular characterisation of ORFV in sheep in Poland. It signals a potential herd biosecurity problem in view of the neglected but re-emerging nature of CE. The marked genetic divergence between Polish field ORFV isolates and currently available vaccine strains may influence CE vaccine efficacy in Poland. Thu, 01 Jan 2026 00:00:00 GMT https://dspace.piwet.pulawy.pl/xmlui/handle/123456789/961 2026-01-01T00:00:00Z https://dspace.piwet.pulawy.pl/xmlui/handle/123456789/960 First record of Hepatospora eriocheir infection in the Chinese Mitten Crab Eriocheir sinensis in the Baltic Sea Stachnik, Magdalena; Normant-Saremba, Monika; Kycko, Anna Hepatospora eriocheir is a microsporidian parasite of the Chinese mitten crab Eriocheir sinensis, an invasive decapod now widely distributed in European inland and coastal waters. Although the host is common across much of Europe, confirmed European records of H. eriocheir have remained scarce and, until now, have not included the Baltic Sea region. In this study, 15 adult E. sinensis collected from the Vistula Lagoon, southern Baltic Sea, were examined using gross pathological assessment, wet-mount microscopy, histopathology, PCR amplification, Sanger sequencing, and phylogenetic analyses of parasite SSU rRNA and host COI sequences. Hepatopancreatic alterations were observed in several individuals, ranging from pale discolouration and friability to loss of normal tissue organisation. Spores were detected in fresh squash preparations from affected tissue, and histology revealed epithelial disruption, intratubular spore accumulation, and necrotic changes consistent with progressive microsporidian infection. Molecular screening confirmed H. eriocheir in 60.0% of crabs, with positive cases occurring in both females and males. The parasite sequences formed a single, well-supported clade and were highly similar to previously reported H. eriocheir sequences from the United Kingdom and China. Host COI sequences represented three mitochondrial haplotypes, indicating that the infection occurred across more than one host mitochondrial haplotype background. These findings constitute the first record of H. eriocheir in E. sinensis from the Baltic Sea and support the hypothesis that infected crabs reaching the Vistula Lagoon are connected with the wider North Sea invasion system rather than an isolated Baltic lineage. Thu, 01 Jan 2026 00:00:00 GMT https://dspace.piwet.pulawy.pl/xmlui/handle/123456789/960 2026-01-01T00:00:00Z